Fig. 3
From: miR-124-3p functions as a tumor suppressor in breast cancer by targeting CBL
CBL is a direct target of miR-124-3p. a qRT-PCR analysis of the expression levels of miR-124-3p in MCF-7 and MDA-MB-231 cells treated with control mimic, miR-124-3p mimic, control antisense or miR-124-3p antisense. b and c Western blot analysis of the expression levels of CBL protein in MCF-7 and MDA-MB-231 cells treated with control mimic, miR-124-3p mimic, control antisense or miR-124-3p antisense. b representative image; c quantitative analysis. d Firefly luciferase reporters containing wild-type (WT) or mutant (MUT) miR-124-3p binding sites in the CBL 3’-UTR were co-transfected into HEK293T cells together with control mimic or miR-124-3p mimic. The cells were assayed using a luciferase assay kit 24 h post-transfection. *P < 0.05; **P < 0.01; ***P < 0.001