Fig. 4
Lapatinib and NH125 exert synergistic effects through the Src and Erk signalling pathways. a, b, c and d CNE-2 and HONE-1 cells were treated with lapatinib, NH125 or their combination for 48 h. a Cell viability was assessed by the CCK-8 assay. b Inhibition of proliferation was measured by the crystal violet assay. c IC50 isobologram of the lapatinib and NH125 combination treatment. In the isobologram, a plot to the left under the line indicates that the combination is synergistic. d Cleaved PARP, phosphorylated AKT, phosphorylated ERK and phosphorylated Src levels were examined by Western blot analysis. GAPDH was used as a loading control