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Fig. 4 | BMC Cancer

Fig. 4

From: Induction of autophagy by ARHI (DIRAS3) alters fundamental metabolic pathways in ovarian cancer models

Fig. 4

ARHI Expression Increases Glutamine Uptake and Glutamate Excretion in SKOv3-ARHI. a Quantitation of 5-13C-glutamine uptake in SKOv3-ARHI between 24 and 48 h in culture by 13C NMR. Uptake was calculated by subtracting the media Gln C5 carbon-13 signal at 48 h from the media signal at 24 h and normalized to the total media proton signal. b Normalized concentration of 13C-labeled extracellular glutamate derived from 5-13C-glutamine after incubation with 5-13C-glutamine for 24 h. Values were obtained by adding the integrated C1 and C5 glutamate resonances and dividing by the total proton signal in the media. Three replicates were used for each condition to calculate the mean and standard deviation. c Effect of Glutaminase inhibition on SKOv3-ARHI and d HEY-ARHI cell viability. BPTES was incubated with cells at the concentrations shown in the presence and absence of Dox induction for 24 and 48 h. Cell viability was determined by MTT assay. Each experimental condition was carried out in triplicate and used to calculate the mean and standard deviation. Statistical significance was determined by two-way ANOVA and p-values adjusted for multiple comparisons (*, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001)

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