Fig. 3
ARHI induction alters glucose and choline metabolism. a SKOv3-ARHI cells were induced with Dox or uninduced for 48 h followed by the addition of 13C6-glucose in the media. The intracellular water-soluble metabolite concentrations were determined by 1H and 13C-filtered 1H NMR spectra (1D HSQC) after 8 h, normalized to cell count, and expressed as a ratio between induced and uninduced samples. b Glycerophosphocholine:phosphocholine (GPC/PC) ratio in water-soluble extracts of SKOv3-ARHI cells transfected with shAtg5 or a control shRNA. Data was obtained by 1H NMR of water soluble metabolite extracts of SKOv3-ARHI between 24 and 48 h in culture. Three replicate samples were used for each condition to calculate the mean and standard deviation and statistical significance was determined by two-way ANOVA with p-values adjusted for multiple comparisons (*, p < 0.05; **, p < 0.01)