Fig. 2From: Identification of BRCA1-like triple-negative breast cancers by quantitative multiplex-ligation-dependent probe amplification (MLPA) analysis of BRCA1-associated chromosomal regions: a validation studyImmunohistochemical PARP1 staining in TNBC tissue microarrays. a b BRCA1-like TNBC with high (3+) nuclear PARP1 levels in tumor cells (10× magnification) as assessed by a pathologist. 3+ stained nuclei are exemplarily indicated by black arrows in a separate image section. c Non-BRCA1-like TNBC with low cytosolic and nuclear PARP1 levels in tumor cells (10× magnification). Black arrow shows an unstained nucleus. Tissue microarrays were incubated with mouse anti-PARP antiserum followed by staining with peroxidase-conjugated secondary antibody molecules and diaminobenzidine (DAB+) as chromogenic substrate. Nuclear counterstaining was performed with hematoxylinBack to article page