Fig. 2

MK-0646 increases Insulin Receptor A Isoform expression in MCF-7/AC-1 xenografts. MCF-7/AC-1 xenograft tumors were harvested and immediately flash frozen following 28 days of treatment (Fig. 1). RNA and protein were extracted for Insulin Receptor and IGF-1R quantification by qPCR (a) and western blot analysis (b) as described in the methods. a Absolute IGF1R (Top) and IRA/B isoform (Bottom) copy number normalized to RPL19 housekeeper. b Protein from pooled group replicates isolated at indicated time points were subject to western blotting with indicated antibodies, as described in Materials and Methods section. Error bars represent SEM. *, P < 0.05; **, P < 0.01; ***, P <0.001; **** P < 0.0001