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Fig. 5 | BMC Cancer

Fig. 5

From: A diagnostic marker for superficial urothelial bladder carcinoma: lack of nuclear ATBF1 (ZFHX3) by immunohistochemistry suggests malignant progression

Fig. 5

Fragments without NLS at the N- and C termini localized to the cytoplasm and fragments with NLS localized to the nucleus. a, Map of four HA-tagged ATBF1 expression vectors. HA-ATBF1 consisted of full-length ATBF1 cDNA. ATBF1-2.7 N consisted of 2.7-kbp of N-terminal cDNA. ATBF1-7.2 M consisted of 7.2-kbp of cDNA from the middle section encoding three NLS sequences exclusively. ATBF1-1.2C consisted of 1.2-kbp of C-terminal cDNA. The HA tag was localized at the N terminus of the ATBF1 expression vectors. The sites of the three NLSs are indicated by red triangles. b, Overexpression studies in HEK293T cells. HA-ATBF1 expressed the HA tag in the nucleus. ATBF1-2.7 N expressed the HA tag in the cytoplasm. ATBF1-7.2 M expressed the HA tag in the nucleus. ATBF1-1.2C expressed the HA tag in the cytoplasm. Scale bar = 5 μm. c, Full-length ATBF1 protein derived from HA-ATBF1 observed mainly in the nucleus. Truncated protein expressed by the N-terminal 2.7-kbp cDNA observed in the cytoplasmic fraction. Truncated protein expressed by the middle 7.2-kbp cDNA observed in the nuclear fraction. Truncated protein expressed by the C-terminal 1.2-kbp cDNA observed in the cytoplasmic fraction. Lamin A is a nuclear marker and α-tubulin is a cytoplasmic marker

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