Fig. 6

P38 and JNK inhibitors afforded protection against CPS-induced apoptosis. a 786-O cells were treated by monotherapy of CPS, or combined therapy of CPS with pretreatment of P38 inhibitor (SB203580, 20 μM) or JNK inhibitor (SP600125, 10 μM) for 4 h, respectively, Then, cell viability was measured by CCK8 assays. b Pretreatment of SB203580 or SP600125 inhibited the caspases activation induced by CPS, which was measured by western blot. c, d and e The results of the western blot were quantified and expressed in histograms. The results were quantitated by densitometric analysis. The Figures are representative of three experiments. The data are presented as mean ± SD. The quantitative data of the western blot and statistic analysis was performed by One-Way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001; bars, SD