Fig. 1

CPS decreased the viability of 786-O cells. a Cell viabilities were determined after 786-O cells was incubated with vehicle (0.1 % DMSO) or different concentrations of CPS for 12, 24, 48 h by CCK8 assay, and are expressed as percent against control, which was taken as 100 %, and treated with medium-containing vehicle (0.1 % DMSO). b Pretreatment of CPZ at 2 μM for 2 h significantly attenuated the decreasing of cell viability by 48 h incubation of CPS. All of the CCK8 assays were conducted in triplicate. c and d The expression of TRPV1 in 786-O, T24, and 5637 cells was detected by RT-PCR (c) and western blot (d). β actin and GAPDH were used as internal standards in RT-PCR and western blot, respectively. e and f TRPV1 expression was quantified and the results of RT-PCR (e) and western blot (f) were presented in histograms. Expression of TRPV1 in different cells was normalized over 786-O cells. *P < 0.05, **P < 0.01, ***P < 0.001; bars, SD. One-Way ANOVA was used for the data analysis