Fig. 6

DEAB-mediated ALDH inhibition affects NB aggressive properties and sensitizes NB cells to 4-hydroxycyclophosphamide. Analyses of the impact of ALDH activity inhibition by DEAB treatment on NB cell proliferation (2D-growth, a), clonogenicity (3D-growth, b), TICs self renewal (c), and sensitivity to 4-HCPA (a-e). SK-N-Be2c and NB1-C cell lines were pre-treated with 100 or 50 μM of DEAB, respectively, for three days before starting functional assays performed in presence of DEAB or DMSO as control. a Mean OD at 405 nm ± SD of 4 (SK-N-Be2c) or 2 (NB1-C) experiments performed in quadruplicates. b Mean of relative colony numbers ± SD of 3 experiments performed in duplicates (unpaired t-test *p < 0.05, ***p ≤ 0.0005). c Mean ratio of cell number/cell plated ± SD of 2 experiments performed in triplicates (unpaired t-test ***p ≤ 0.0001). d Cell viability of SK-N-Be2c and NB1-C cells treated for 48 h with indicated doses of 4-HCPA in presence or absence of DEAB (100 μM and 50 μM, respectively). Mean values ± SD of 3 experiments performed in quadruplicates (unpaired t-test *p < 0.02, ***p ≤ 0.0001). e Cell viability of IGR-N91 and IGR-N91R cells treated for 48 h with indicated doses of 4-HCPA in presence or absence of DEAB (100 μM). Mean values ± SD of 3 experiments performed in quadruplicates (unpaired t-test ***p ≤ 0.0005)