Fig. 6From: Hepatocellular carcinoma-targeted drug discovery through image-based phenotypic screening in co-cultures of HCC cells with hepatocytesPyrimethamine has anticancer effects against Huh7 cells in a 3D culture system. Fluorescence images of cleaved caspase-3, which is an apoptosis marker, and F-actin, which detects bile canaliculus structures, in (a) Fa2N-4 and (b) Huh7 spheroids after pyrimethamine treatment (10 μM, 7 days). c Schematic illustrations of mechanisms by which pyrimethamine induces HCC-specific toxicity based on the results. Scale bar = 200 μmBack to article page