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Fig. 4 | BMC Cancer

Fig. 4

From: Hepatocellular carcinoma-targeted drug discovery through image-based phenotypic screening in co-cultures of HCC cells with hepatocytes

Fig. 4

Pyrimethamine induces DNA damage and S-phase arrest, but does not affect ROS accumulation and mitochondrial dysfunction. a Immunocytochemistry images of γ-H2AX in Huh7 cells after 24 h incubation with the anti-folate drugs at a concentration of 10 μM. b Intensity of γ-H2AX in the Fa2N-4 and Huh7 cell lines after treatment with pyrimethamine at the indicated concentrations. Experiments were performed in triplicate. Error bars indicate standard deviation. c Analysis of proliferation activity in Huh7 and Fa2N-4 cells using EdU staining after 24 h incubation with pyrimethamine at the indicated concentrations. d ROS and mitochondrial membrane potential (MMP) were measured after 24 h incubation with anti-folate drugs. ROS and MMP were detected by staining with CM-H2DCFDA (green) and tetramethylrhodamine, methyl ester (TMRM; red). All images and analyses were examined using the High Content Screening System. Images in the same panel were obtained with same magnification

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