Fig. 3

Identification of compounds that are selective for HCC. a Schematic of screening of HCC selective compounds in a mixed culture system (Fa2N-4 and Huh7 cells) and screening data. 0.01 % of DMSO was used as negative control (red circle), 10 μM of sorafenib was used as positive control (green circle) and hit compounds (Blue circle). b Schematic of the drug discovery process, from pilot screening to the mechanism of action study. c Immunofluorescence images of the mixed culture system after treatment with DMSO and 10 μM of pyrimethamine. Images were obtained with same magnification. d Viability of Huh7 (CHALV1⁺/AFP⁺) and Fa2N-4 (CHALV1⁻/AFP⁻) cells in the mixed culture system using image-based analysis. Experiments were performed in triplicate. Error bars indicate standard deviation