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Fig. 2 | BMC Cancer

Fig. 2

From: Hepatocellular carcinoma-targeted drug discovery through image-based phenotypic screening in co-cultures of HCC cells with hepatocytes

Fig. 2

CHALV1 and AFP are cancer-specific markers for HCC cells. a Immunocytochemistry images of CHALV1 and AFP in primary hepatocytes (83857) and primary HCC cells, which were dissociated from liver resection specimens of liver cancer patients. b Representative immunohistochemistry images of CHALV1 and AFP in liver cancer tissues and surrounding normal tissues. c Expression of CHALV1 and AFP in hepatocyte (Fa2N-4), hepatoblastoma (Huh6), and HCC cell lines. d Fluorescence images of AFP and CHALV1 in a mixed culture system with CellLight® Nucleus-GFP-labeled Fa2N-4 cells (sky blue) and Huh7 cells. All images and graphs were analyzed using the Operetta® High Content Screening System. Images in the same panel were obtained with same magnification

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