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Fig. 5 | BMC Cancer

Fig. 5

From: The histone deacetylase inhibitor trichostatin a decreases lymphangiogenesis by inducing apoptosis and cell cycle arrest via p21-dependent pathways

Fig. 5

p21, but not p27 and p53, mRNA expression is upregulated by TSA. a Semiquantitative RT-PCR analyses for p21, p27, p53 and β-Actin as loading control were performed on total RNA extracted from subconfluent LECs. LECs were left untreated (solvent only, ethanol), or were treated with TSA (at 400 nM) for the indicated times. Results were confirmed in three independent sets of experiments. b LECs were incubated in the presence or absence of TSA (400 nM) for 3 h and incubated for 0, 1, 3 and 6 h with fresh media containing actinomycin D (10 μg/ml). Then, p21 mRNA levels were quantified using quantitative PCR. Comparable results were obtained from three independent experiments. c LECs were incubated in the presence or absence of TSA (400 nM) for 3 h and incubated for 0, 1, 3 and 6 h with fresh media containing cycloheximide (10 μg/ml). p21 and α-tubulin protein were detected by enhanced chemiluminescence. p21 protein remaining at these time points was determined by densitometric scanning, the results of which were normalized to amounts of α-tubulin. Comparable results were obtained from three independent experiments

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