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Fig. 4 | BMC Cancer

Fig. 4

From: The histone deacetylase inhibitor trichostatin a decreases lymphangiogenesis by inducing apoptosis and cell cycle arrest via p21-dependent pathways

Fig. 4

TSA mediates nuclear accumulation of p21 protein in LECs. Subconfluent LECs were either left untreated (solvent only) or treated with 400 nM TSA for 24 h. Cells were fixed and stained with anti-p21 antibody. Blue: Hoechst stained nuclei; red: p21 protein stained with specific antibody and secondary antibody conjugated with Alexa Fluor 546. Comparable results were obtained from three independent experiments

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