Fig. 2

TSA induces G0/G1 cell cycle arrest in LECs. a Cells were incubated with 400 nM TSA and ethanol as a control for 24 h after blocking the S-phase of the cell-cycle by treatment with a serum depleted-medium for 24 h. Analysis of cell cycle as assessed by FACS using propidium iodide-stained LECs. The figure shows the percentage of cells in G0/G1, S and G2/M with regard to solvent controls (Ethanol). Data displayed are representative of eight experiments that were performed revealing comparable results. *p < 0.05 vs Ctrl. b, c and d Representative western blot analyses of LECs that were left untreated (solvent only) or were treated with TSA in a concentration- and time-dependent manner. Cell cycle control related proteins, like p21, p27, p53, cyclin A/D1, CDK4, CDK6 and α-Tubulin as loading control were detected by enhanced chemiluminescence. Comparable results were obtained from three independent experiments