Fig. 8

Hemangiomas in knockout mice are partially rescued by reconstitution with wild-type hematopoietic stem cells. Hematopoietic stem cells were enriched by sorting Hoechst negative side population (SP) from bone marrow. 8–9-week old wild-type mice or 4-week old HOXB7-Cre; Vhlh^fl/fl mice were lethally irradiated and transplanted with 1000 SP obtained from 2 to 3 month old HOXB7-Cre; Vhlh^fl/fl mice (KO), or Cre-negative Vhlh^fl/fl littermates (WT). a Representative Hoechst stain followed by sorting. Percentage of Hoechst negative cells is indicated. Only the tip of the Hoechst negative tail that contains cells with the least Hoechst fluorescence was collected. b Representative flow data of peripheral blood leukocytes stained with CD45.1 and CD45.2 two months after the transplant (wild-type recipients) or one month after the transplant (HOXB7-Cre; Vhlh^fl/fl recipients). Recipients were CD45.1/2 double positive and were transplanted with CD45.1 single positive SP. c Summary of transplant experiments. d–i Representative phenotypes of the transplanted chimera. Livers of KO recipients were obtained one month after the transplant, at ~2 months of age; livers of WT recipients were obtained at 2, 3 and 6 months after transplantation. Numbers beneath images indicate number of mice with phenotype versus total number of mice analyzed. d Wild-type SP to wild-type recipients (WT - > WT) did not show any phenotype. e Knockout SP to knockout recipients (KO - > KO) showed full-penetrant phenotype. f–h Hemangiomas are partially rescued in HOXB7-Cre; Vhlh^fl/fl recipient mice (KO) transplanted with SP from Cre negative Vhlh^fl/fl mice (WT). i Knockout SP to wild-type recipients (KO - > WT) showed no phenotype