Fig. 5

miR-10b binds to CSMD1 3’-UTR and represses CSMD1 expression in HepG2 cells. a Luciferase activity assay. HepG2 cells were co-transfected with pMIR/CSMD1 3’-UTR or mutated pMIR mu-CADM1 3’-UTR plus miR-10b mimics or negative control (NC). miR-10b overexpression markedly suppressed luciferase expression in HepG2 cells transfected with pMIR-CSMD1-3’-UTR-WT but not pMIR-CSMD1-3’-UTR-mut1. b qRT-PCR. Expression of CSMD1 mRNA after transfection with hsa-miR-10b mimics (10b-m) or hsa-miR-10b inhibitors (10b-i) was measured by qRT-PCR in HepG2. miR-10b overexpression decreased CSMD1 expression; miR-10b knockdown increased CSMD1 expression in cultured cells c Immunocytochemistry. CSMD1 expression was low in HepG2 compared to normal liver HL-7702cells. d Immunocytochemistry. CSMD1 expression was higher in HepG2 cells transfected with the miR-10b inhibitors, whereas CSMD1 expression was reduced in HepG2 cells transfected with miR-10b mimics