Fig. 3

Knockdown of IGF-1R reduces the proliferation of resistant cells. a Relative expression of EGFR and IGF1R in resistant cells subjected to knockdown with three independent siRNAs targeting EGFR or IGF1R respectively after 60 h. Scrambled control was set to 1. b as in A but for the sensitive cell line. c Representative western blot indicating reduced levels of EGFR (left panel) or IGF1R (right panel) after knockdown with siRNA #1 and #8 respectively in resistant cells. GAPDH was used as a loading control protein was quantified at 60 h. d As in C but for the sensitive cell line. e Proliferation of resistant and sensitive cell lines in untreated, scrambled and specific knockdown conditions, measured using the CCK-8 assay, measured at 72 h. IGF1R has a greater effect on proliferation in resistant as compared to sensitive cells