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Fig. 6 | BMC Cancer

Fig. 6

From: Selective depletion of tumour suppressors Deleted in Colorectal Cancer (DCC) and neogenin by environmental and endogenous serine proteases: linking diet and cancer

Fig. 6

Transfection of dcc into cultured cells. A photomicrograph of normal SH-SY5Y cells after incubation with antibodies to DCC showed only very weak immunofluorescence (a) whereas 48 h after transient transfection with a dcc plasmid, the fluorescence was substantially greater (b) and no image was obtained in the absence of DCC antibody (c). The fluorescence images are consistent with immunoblots for DCC protein which indicated an approximately 50-fold increase in expression (d). The protein was down-regulated by subtilisin (d) (30 and 100nM) as observed previously in adult tissue. The relative expression of neogenin (e) or RhoA (f) was unchanged by dcc transfection and neogenin was reduced by subtilisin while RhoA remained unaffected, as in the tissue work. An exactly similar pattern was seen in transiently transfected MCF-7 cells, with a profound reduction by subtilisin of ectopic DCC expression (g) and no change in RhoA (h). Bars represent mean ± s.e.mean (n = 4–6). *P < 0.05; **P < 0.01; relative to the corresponding control bar. Scale bar: 50 μm

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