Fig. 6

Effect of ATG7-specific siRNAs (Atg7-1 or Atg7-3) or a scrambled control siRNA (Scramble) on doxorubicin sensitivity and expression of Atg7 protein in MCF-7CC10 and MCF-7DOX2–10 cells. Doxorubicin sensitivity was assessed using clonogenic assays (a), while the efficiency of gene knockdown was assessed in immunoblotting experiments (b) using antibodies specific for the Atg7 protein. A γ-tubulin antibody was used as a loading control