Fig. 7
Hypoxia promotes anti-apoptotic signals in leukemic cells independently of chemotherapies. a Effect of hypoxia (5 % O2) over 48 h on nuclear expression of HIF-1α protein in Nalm-6 cells. b Nalm-6 and Reh cells were cultured either in normoxia or hypoxia and untreated or treated with MTX or PRD for 3 h and lysed. Equal amounts of proteins were fractionated by SDS-PAGE and Western blotted with anti-Mcl-1, anti-Bcl-2, anti-Bim and anti-β actin. c Western blotting quantification was performed on the image from Fig. 7B