Fig. 4

Effects of the hsa-miR-17-5p and hsa-miR-20a-5p mirVana® miRNA inhibitors. a Effects of miRNA inhibitors indicated by ①-③ on the expression levels of miR-17-5p and -20a-5p. b Effects of miRNA inhibitors indicated by ①-④ on the expression of HDPA8 and p21. The expression of HDPA8 and P21 were examined by both qPCR and western blotting. The graph shown under the western blotting images indicates the band densitometry results. c Cell cycle analysis after the transfection of cells with miRNA inhibitors. The ratios of each cell cycle phase are shown as a bar graph. Blue color indicates G1 phase, red color indicates S phase, and green color indicates the G2/M phase cell ratio. Error bars indicate the standard error (SE) of four different experiments. In the case of western blotting, the SE was calculated from the densitometry data. *p < 0.05, **p < 0.01, ***p < 0.001 (n = 4 each)