Fig. 2
From: microRNA-mediated resistance to hypoglycemia in the HepG2 human hepatoma cell line
The expression levels of miR-15b-5p and −16-5p and their target gene HSPA1B in HepG2 cells after incubation with various concentrations of glucose. Cells were cultured with 200, 900, and 1800 mg/L of glucose for 1 week and qPCR and western blotting were performed to quantitate the expression of miRNAs and their target mRNA and protein. a The expression levels of mRNA and protein of HSPA1B. The graph shown under the western blotting images indicates the results of band densitometry analysis. b Expression levels of miR-15b-5p and miR-16-5p. Error bars indicate the standard error (SE) of four different experiments. In the case of western blotting, the SE was calculated from the densitometry data. *p < 0.05 (n = 4 each)