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Fig. 5 | BMC Cancer

Fig. 5

From: Iterative sorting reveals CD133+ and CD133- melanoma cells as phenotypically distinct populations

Fig. 5

CD133- cells derived from CD133+ cells have a CD133+ phenotype. a Sort 3 CD133+ (green), and CD133- (red) cells were re-sorted and CD133- cells from the CD133+ population collected (CD133-(+derived), blue). Sub-cutaneous tumours were established from these 3 populations (2 mice per group) and excised at ~200 mm3. Flow cytometry analysis of CD133 surface expression was performed on viable cells at 2 h post-excision. Signal from isotype control antibody, grey. b CD133+ (green), CD133- (red) and CD133-(+derived) (blue) tumours were collected and CD133 transcript in each tumour quantified by microarray analysis. Data representative of 2 replicate experiments

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