Fig. 5
Anchorage dependent and independent growth with CD44 or RHAMM loss in bladder cancer cells +/− AGL. a–d 72 h after UMUC3 or T24T shCTL and shAGL were transfected with siCTL, siCD44 or siRHAMM, they were plated for monolayer growth (n = 6) in 96-welled plate (103 cells/well) for 5 days followed by CyQUANT assay. e, f 72 h after UMUC3 or T24T shCTL and shAGL were transfected with siRNA against CD44 (siCD44) or RHAMM (siRHAMM) they were plated in agar for evaluation of anchorage independent growth (15x103 cells/well) in 6 well plate (n = 3). Results are shown as mean ± SD, *P < 0.05