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Fig. 4 | BMC Cancer

Fig. 4

From: CD44 and RHAMM are essential for rapid growth of bladder cancer driven by loss of Glycogen Debranching Enzyme (AGL)

Fig. 4

TUNEL assay to detect apoptosis with HAS2, CD44 or RHAMM loss in bladder cancer cells +/− AGL. a, b UMUC3 and T24T shCTL and shAGL cells were plated in chambered slides and 24 h later transfected with scrambled siRNA or siRNA against HAS2 (siHAS2), CD44 (siCD44) or RHAMM (siRHAMM). Details of siRNA are in Material and Methods. 48 h after transfection cells were subjected to TUNEL assay according to manufacturer protocol as mentioned in Material and Methods. Images were taken at a 40X magnification using Olympus IX71 microscope. c, d Quantification of TUNEL staining in UMUC3 and T24T shCTL and shAGL cells with the different gene knockdowns (n = 3). TUNEL staining quantified using ImageJ. Results are shown as mean ± SD, *P < 0.05

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