Fig. 3

RHAMM loss and apoptosis in bladder cancer cells +/− AGL. a UMUC3 shCTL and shAGL cells were plated and 24 h later transfected with scrambled siRNA (siCTL) or siGENOME SMARTpool siRNA against RHAMM (siRHAMM). Details of siRNA are in Material and Methods. Cells were lysed 48 h after transfection and Western blot was carried out for proteins involved in apoptosis. b Densitometric analysis of cleaved apoptotic proteins normalized to total protein and the UMUC3 shCTL siCTL sample (n = 3). DR5 and Fas normalized to Actin and the UMUC3 shCTL siCTL sample (n = 3). Results are shown as mean ± SD, *P < 0.05. c T24T shCTL and shAGL cells were plated and 24 h later transfected with scrambled siRNA (siCTL) or siGENOME SMARTpool siRNA against RHAMM (siRHAMM). Details of siRNA are in Material and Methods. Cells were lysed 48 h after transfection and Western blot was carried out for proteins involved in apoptosis. d Densitometric analysis of cleaved apoptotic proteins normalized to total protein and the T24T shCTL siCTL sample (n = 3). DR5 and Fas normalized to Actin and the UMUC3 shCTL siCTL sample (n = 3). Results are shown as mean ± SD, *P < 0.05