Fig. 1

HAS2 loss and apoptosis in bladder cancer cells +/− AGL. a, b qRT-PCR demonstrating efficacy of HAS2 depletion in UMUC3 and T24T control (shCTL) and AGL knockdown (shAGL) cells. Cells were plated and 24 h later transfected with scrambled (siCTL) or directed siRNA against HAS2 (siHAS2). Details of siRNA used are in Materials and Methods. Cells were harvested at 48 h for mRNA followed by qRT-PCR analysis (n = 3). c 48 h after UMUC3 shCTL and shAGL cells were transfected with scrambled siRNA (siCTL) or siRNA against HAS2 (siHAS2), cells were lysed and expression of CD44, RHAMM and the proteins involved in the apoptotic pathway were detected by Western blot. d Densitometric analysis of cleaved apoptotic proteins normalized to total protein and the UMUC3 shCTL siCTL sample (n = 3). e 48 h after T24T shCTL and shAGL were transfected with scrambled siRNA (siCTL) or siRNA against HAS2 (siHAS2), cells were lysed and expression of CD44, RHAMM and the proteins involved in the apoptotic pathway were detected by Western blot. f Densitometric analysis of cleaved apoptotic proteins normalized to total protein and the T24T shCTL siCTL sample (n = 3). Results are shown as mean ± SD, *P < 0.05