Fig. 3

ATM inhibition enhanced Olaparib induced apoptosis in MCF-7 and CAL-51 cells a, b, DAPI staining indicated the nuclear morphological changes of CAL-51 cells (a) and MCF-7 cells (b). Cells were treated with 0, 10 μM Olaparib with or wihout 10 μM KU55933 respectively for 48 h. Left, representative pictures; right, quantitative data. c, d, Annexin V/PI staining and FACS analysis revealed cell apoptosis of CAL-51 cells (c) and MCF-7 cells (d). Cells were treated with 0, 10 μM Olaparib with or wihout 10 μM KU55933 respectively for 48 h. Left, representative pictures; right, quantitative data. e, Western Blots showed that caspase 3, caspase 8, caspase 9 and their cleaved forms were increased after treatment with 10 μM Olaparib or 10 μM KU55933 or their combination in CAL-51 cells and MCF-7 cells. All experiments were performed at least three times and data were statistically analyzed by two-tail t-test.*p < 0.05, **p < 0.01. Error bars indicate S.E.M