Fig. 2

Treatment of Olarparib, Ku-55933 or their combination induced γ-H2AX foci formation A, B, CAL-51 cells (a) and MCF-7 cells (b) were treated with 0, 5, 10 μM Olaparib with or wihout 10 μM KU55933 respectively for 48 h and analyzed by immunofluorescence (IF) with γH2AX antibody. DAPI was used to visualize cell nuclei. Representative pictures (left) and quantitative data (right) of the γ-H2AX foci. c, CAL-51 cells (left) and MCF-7 cells (right) were treated with 0, 10 μM Olaparib with or wihout 10 μM KU55933 respectively 48 h and analyzed by Western Blot assay (WB) with γH2AX antibody. All experiments were performed at least three times and data were statistically analyzed by two-tail t-test. ***p < 0.001. Error bars indicate S.E.M