Fig. 4
Three-dimensional culture resulted in dramatically enhanced survival of luminal-type breast cancer cells in a TG2-dependent manner. a MCF7_Cont and MCF7_TG2 cells were grown in 3D culture conditions in the presence or absence of IL-1β (10 ng/ml). b MCF7_TG2 cells were grown in 3D culture conditions in the presence or absence of IL-1β (10 ng/ml) and anti-IL-6 monoclonal antibody (10 μg/ml). c MCF7_Cont and MCF7_TG2 cells (1 × 106 cells/each mouse) were injected into the fat pads of NSG mice. Primary tumor growth was measured. Blocking anti-IL-6 antibody (100 μg/mouse) or blocking anti-IL-1β antibody (100 μg/mouse) was injected intraperitoneally every third day, starting 1 day after tumor inoculation. The TG2 inhibitor cysteamine (CyM, 40 mg/kg/day) was injected intraperitoneally starting 1 day after tumor inoculation. Data are given as mean ± SEM of six mice for each group