Fig. 2

IL-1β induced IL-6 production from breast cancer cells in a TG2-dependent manner. a TG2-overexpressing MCF7 cells (TG2) and control vector-transfected MCF-7 cells (Cont) were treated with various cytokines (10 ng/ml) for 48 h and IL-6 levels in culture supernatants were measured by ELISA. b Cells were treated with IL-1β (10 ng/ml) in the presence of TGFβ (10 ng/ml), EGF (10 ng/ml), or TNFα (10 ng/ml) for 48 h and secreted IL-6 levels in culture supernatants were measured by ELISA. c Cells were treated with IL-1β (10 ng/ml) for the indicated times. d Cells were treated with IL-1β at various concentrations for 48 h. a-d All data shown are representative of three independent experiments. Data are presented as mean ± SD