Fig. 6

Orally administered DFX reduced ferritin-H protein levels of removed tumors in immunohistochemical analyses. a Immunohistochemistry was performed on the removed tumors with antibody specific to ferritin-H. b The slides were scored for the percentage of positive cells (0 = 0–5, 1 = 6–25, 2 = 26–50, 3 = 51–75 and 4 = 76–100 %) and intensity (0 = negative, 1 = weak, 2 = moderate, 3 = strong). The immunoreactivity score was calculated as the percentage of positive cells multiplied by the score for the staining intensity. The immunoreactivity scores of removed tumors treated orally with 160 and 200 mg/kg of DFX were significantly lower than that of control tumors. The data are presented as the mean ± SD (n = 5 mice per group). For statistical analysis, each treatment was compared with the control. *p <0.05, **p <0.01 vs. control