Fig. 3
From: In vivo bioluminescence imaging for leptomeningeal dissemination of medulloblastoma in mouse models
Bioluminescence imaging (BLI) and histological analysis for the evaluation of medulloblastoma (MB) seeding. (A and B) After injection of the MB-effLuc cells (UW426, 1.2 × 106; D283, 1.2 × 106; MED8A, 3.0 × 105), firefly luciferase BLI was evaluated and quantified at the indicated days. (C) Representative longitudinal sections of the brains and spinal cords of the mice with saline (control) or MB-effLuc cells (hematoxylin & eosin stain) (1.25×). (D) Inlet figures denote the subfrontal area (a), cerebellum (b), upper thoracic spinal cord (c), lower thoracic spinal cord (d), and conus medullaris (e). Scale bars represent 50 μm. (E) Representative immunofluorescence images (DAPI: blue, Human Nuclei: green, Ki67: red) show that the MB-effLuc cells are highly proliferative in vivo. Scale bars represent 50 μm