Fig. 2

Multi-parameter apoptosis analysis of baicalein-treated H-460 cells. Morphologic features of apoptosis were identified in-vitro by High Content Screening analysis. Apoptosis was induced in a dose-dependent manner after treatment with 100 nM, 1 μM, 10 μM and 100 μM concentrations of baicalein when compared to control cells. 10 μM cisplatin was used as a positive apoptosis control. 3 spectrally distinct fluorophore labels were used to assess cell health by examining nuclei, f-actin (cytoskeletal protein) and mitochondrial potential. Loss of f-actin (green) shows the loss of cell integrity during apoptosis as membrane blebbing occurs and mitochondrial activity increases during apoptosis (orange) coupled with an increase in nuclear condensation