Fig. 6

Influence of SEC62-overexpression and SEC62-silencing in HeLa cells on the expression level of EMT markers. a Immunofluorescence targeting Sec62 (left column, green), F-actin (middle left column, green) and vimentin (middle right column, red) in HeLa cells transfected with control siRNA, SEC62 siRNA, a control plasmid or a SEC62 plasmid. The nuclei of the cells are marked with DAPI (blue signal). b Cellular protein levels of E-cadherin, vimentin, Sec62 and β-actin were quantified in identically pretreated cells and normalized to GAPDH. MCF-7 cells were used as a positive for E-cadherin expression. The relative expression of vimentin, Sec62 and β-actin is indicated below the respective bands as mean value of 4 identically performed experiments (n = 4) with the respective standard error. Images in (a) are shown in 60× magnification. The grey scale bars indicate 20 μm