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Table 4 In total, 22 proteins with differential expression (2-fold increase or decrease, ANOVA p < 0.05) between A2780cisR and the parent A2780 cell line due to treatment with CS in two aliquots with a time gap of 2 h using fresh/fresh and fresh/aged combinations were identified by MALDI-TOF/TOF

From: Changes in the in vitro activity of platinum drugs when administered in two aliquots

Spot ID Full name MALDI location Expression in A2780cisR Post treatment expression Function Tumour association
CS CS + CS
  fresh aged
3 COX5A Cytochrome c oxidase subunit 5A, mitochondrial Score: 63 Mass: 16752 pI: 6.30 Coverage: 11 % Mitochondria Down Regulated PR PR OR Ribosome biogenesis; mitochondrial respiratory chain Breast [77]; cervix [78]; colon [77]; gastric [31]; Kidney [79]; Lung [8082]; Nasopharyngeal [32]; Oesophageal [77]; Ovarian [77]; Prostate [77]; Thyroid [83]
19 COF2 Cofilin‐2 Score: 127 Mass: 18725 pI: 7.66 Coverage: 39 % Cytoplasm, cytoskeleton Down Regulated R R OR Actin polymerization Pancreatic [84]; Prostate [85]
34 1433G 14‐3‐3 protein gamma Score: 315 Mass: 28285 pI: 4.80 Coverage: 44 % Cytoplasm Down Regulated PR PR OR Adapter protein Breast [86]
41 MARE1 Microtubule‐associated protein RP/EB family member 1 Score: 62 Mass: 29980 pI: 5.02 Coverage: 29 % Cytoplasm Down Regulated - R OR Microtubule cytoskeleton dynamics; cell migration. Colon [87]; lung [88]; gastric [89]; oesophageal [90];
50 NPM Nucleophosmin Score: 68 Mass: 32555 pI: 4.64 Coverage: 26 % Nucleoplasm Down Regulated - R OR Chaperone; ribosome biogenesis; p53 and ARF regulation Bladder [91]; breast [92]; Colon [93]; gastric [94]; haematopoietic [40]; ovarian [95]; prostate [96]
51 ANXA1 Annexin A1 Score: 369 Mass: 38690 pI: 6.57 Coverage: 53 % Nucleus, cytoplasm Down Regulated R R OR Calcium/phospholipid-binding protein breast [97]; gastric [98]; HNSC [36]; lung [99]; oesophageal [100, 101]; prostate [100, 102];
62 RSSA 40S ribosomal protein SA Score: 311 Mass: 32833 pI: 4.79 Coverage: 34 % Cell membrane, cytoplasm, nucleus Up Regulated R R - laminin receptor; fate determination; tissue morphogenesis Breast [103]; cervical [104]; colon [54, 55, 105]; melanoma [106]
65 ACTB Actin, cytoplasmic 1 Score: 767 Mass: 41710 pI: 5.29 Coverage: 53 % Cytoplasm Up Regulated PR R PR cell motility Colon [107]; gastric [108]; liver [109]; sarcoma [110]
70 CALU Calumenin Score: 141 Mass: 37084 pI: 4.47 Coverage: 14 % Sarcoplasmic, endoplasmic reticulum Up Regulated - OR PR Vitamin K-dependent carboxylation Colon [111]; gastric [112]; glioblastoma [113]; prostate [114];
72 HNRPF Heterogeneous nuclear ribonucleoprotein F Score: 342 Mass: 45643 pI: 5.38 Coverage: 33 % Nucleus Down Regulated R OR R processing of pre-mRNAs; alternative splicing events Gastric [115]; thyroid [116]
76 ENOA Alpha‐enolase Score: 471 Mass: 47139 pI: 7.01 Coverage: 47 % Cytoplasm Down Regulated - PR R Glycolysis; hypoxia tolerance; tumour suppressor Breast [117]; lung [118]; nasopharyngeal [119];
85 ATPA ATP synthase subunit alpha, mitochondrial Score: 391 Mass: 59714 pI: 9.16 Coverage: 29 % Mitochondria Up Regulated - OR OR Production of ATP from ADP Breast [120]; colon [121]; gastric [122]; leukemia [123]; thyroid [124];
92 PDIA3 Protein disulfide-isomerase A3 Score: 525 Mass: 56747 pI: 5.98 Coverage: 54 % Endoplasmic reticulum Down Regulated PR PR R endopeptidase; electron carrier; Lung [125]; ovarian [48]; Prostate [46]; retinoblastoma [126]
100 HNRPK Heterogeneous nuclear ribonucleoprotein K Score: 123 Mass: 50944 pI: 5.39 Coverage: 26 % Cytoplasm, nucleus Up Regulated - OR - Pre-mRNA-binding proteins; p53 response to DNA damage Breast [127]; colon [128]; Leukemia [129, 130]; lung [131]; Oropharyngeal [132]; pancreatic [133];
103 CH60 60 kDa heat shock protein, mitochondrial Score: 762 Mass: 61016 pI: 5.70 Coverage: 39 % Mitochondria Up Regulated PR OR PR macromolecular assembly; stress working chaperone Bronchus [134]; Colon [135]; leukemia [136]; prostate [137]
104 TCPQ T‐complex protein 1 subunit theta Score: 200 Mass: 59583 pI: 5.42 Coverage: 24 % Cytoplasm Down Regulated R OR R chaperone Colon [138]; liver [139];
127 GRP75 Stress‐70 protein, mitochondrial Score: 839 Mass: 73635 pI: 5.87 Coverage: 33 % Mitochondria, nucleus Down Regulated R PR R Cell proliferation; cellular aging; chaperone Brain, Breast, colon, kidney, lung, ovarian [140]
139 GLU2B Glucosidase 2 subunit beta Score: 80 Mass: 59388 pI: 4.33 Coverage: 11 % Endoplasmic reticulum Up Regulated OR OR - Regulatory subunit of glucosidase II Colon [138, 141]; sarcoma [142];
149 HSP74 Stress‐70 protein, mitochondrial Score: 187 Mass: 94271 pI: 5.11 Coverage: 10 % Cytoplasm Down Regulated - OR PR Stress response; cell proliferation, differentiation Breast [143]
170 HNRPC Heterogeneous nuclear ribonucleoproteins Score: 142 Mass: 33650 pI: 4.95 Coverage: 8 % Nucleus Up Regulated - R OR mRNA processing and translation Breast [144]; colon [145]; lung [146]; pancreatic [147];
215 CALR Calreticulin Score: 246 Mass: 48112 pI: 4.29 Coverage: 17 % Endoplasmic, sarcoplasmic reticulum Up Regulated - R OR Calcium-binding chaperone Bladder [69]; breast [148]; colon [149]; gastric [150]; glioblastoma [69]; liver [151]; ovarian [69]; pancreas [152]; prostate [153]
246 VIME Vimentin Score: 539 Mass: 53619 pI: 5.06 Coverage: 81 % Cytoplasm Up Regulated P OR - Filaments attached to nucleus and endoplasmic reticulum Breast [154]; gastric [155]; lung [156]; pancreas [157]
  1. (−) indicates that the spot was either not visible on the gel or that the treatment had no effect on the expression of the protein
  2. R Restored, PR Partially Restored, OR Over Restored