Table 4 In total, 22 proteins with differential expression (2-fold increase or decrease, ANOVA p < 0.05) between A2780^cisR and the parent A2780 cell line due to treatment with CS in two aliquots with a time gap of 2 h using fresh/fresh and fresh/aged combinations were identified by MALDI-TOF/TOF

3

COX5A

Cytochrome c oxidase subunit 5A, mitochondrial

Score: 63

Mass: 16752

pI: 6.30

Coverage: 11 %

Mitochondria

Down Regulated

PR

PR

OR

Ribosome biogenesis; mitochondrial respiratory chain

Breast [77]; cervix [78]; colon [77]; gastric [31]; Kidney [79]; Lung [80–82]; Nasopharyngeal [32]; Oesophageal [77]; Ovarian [77]; Prostate [77]; Thyroid [83]

19

COF2

Cofilin‐2

Score: 127

Mass: 18725

pI: 7.66

Coverage: 39 %

Cytoplasm, cytoskeleton

Down Regulated

R

R

OR

Actin polymerization

Pancreatic [84]; Prostate [85]

34

1433G

14‐3‐3 protein gamma

Score: 315

Mass: 28285

pI: 4.80

Coverage: 44 %

Cytoplasm

Down Regulated

PR

PR

OR

Adapter protein

Breast [86]

41

MARE1

Microtubule‐associated protein RP/EB family member 1

Score: 62

Mass: 29980

pI: 5.02

Coverage: 29 %

Cytoplasm

Down Regulated

-

R

OR

Microtubule cytoskeleton dynamics; cell migration.

Colon [87]; lung [88]; gastric [89]; oesophageal [90];

50

NPM

Nucleophosmin

Score: 68

Mass: 32555

pI: 4.64

Coverage: 26 %

Nucleoplasm

Down Regulated

-

R

OR

Chaperone; ribosome biogenesis; p53 and ARF regulation

Bladder [91]; breast [92]; Colon [93]; gastric [94]; haematopoietic [40]; ovarian [95]; prostate [96]

51

ANXA1

Annexin A1

Score: 369

Mass: 38690

pI: 6.57

Coverage: 53 %

Nucleus, cytoplasm

Down Regulated

R

R

OR

Calcium/phospholipid-binding protein

breast [97]; gastric [98]; HNSC [36]; lung [99]; oesophageal [100, 101]; prostate [100, 102];

62

RSSA

40S ribosomal protein SA

Score: 311

Mass: 32833

pI: 4.79

Coverage: 34 %

Cell membrane, cytoplasm, nucleus

Up Regulated

R

R

-

laminin receptor; fate determination; tissue morphogenesis

Breast [103]; cervical [104]; colon [54, 55, 105]; melanoma [106]

65

ACTB

Actin, cytoplasmic 1

Score: 767

Mass: 41710

pI: 5.29

Coverage: 53 %

Cytoplasm

Up Regulated

PR

R

PR

cell motility

Colon [107]; gastric [108]; liver [109]; sarcoma [110]

70

CALU

Calumenin

Score: 141

Mass: 37084

pI: 4.47

Coverage: 14 %

Sarcoplasmic, endoplasmic reticulum

Up Regulated

-

OR

PR

Vitamin K-dependent carboxylation

Colon [111]; gastric [112]; glioblastoma [113]; prostate [114];

72

HNRPF

Heterogeneous nuclear ribonucleoprotein F

Score: 342

Mass: 45643

pI: 5.38

Coverage: 33 %

Nucleus

Down Regulated

R

OR

R

processing of pre-mRNAs; alternative splicing events

Gastric [115]; thyroid [116]

76

ENOA

Alpha‐enolase

Score: 471

Mass: 47139

pI: 7.01

Coverage: 47 %

Cytoplasm

Down Regulated

-

PR

R

Glycolysis; hypoxia tolerance; tumour suppressor

Breast [117]; lung [118]; nasopharyngeal [119];

85

ATPA

ATP synthase subunit alpha, mitochondrial

Score: 391

Mass: 59714

pI: 9.16

Coverage: 29 %

Mitochondria

Up Regulated

-

OR

OR

Production of ATP from ADP

Breast [120]; colon [121]; gastric [122]; leukemia [123]; thyroid [124];

92

PDIA3

Protein disulfide-isomerase A3

Score: 525

Mass: 56747

pI: 5.98

Coverage: 54 %

Endoplasmic reticulum

Down Regulated

PR

PR

R

endopeptidase; electron carrier;

Lung [125]; ovarian [48]; Prostate [46]; retinoblastoma [126]

100

HNRPK

Heterogeneous nuclear ribonucleoprotein K

Score: 123

Mass: 50944

pI: 5.39

Coverage: 26 %

Cytoplasm, nucleus

Up Regulated

-

OR

-

Pre-mRNA-binding proteins; p53 response to DNA damage

Breast [127]; colon [128]; Leukemia [129, 130]; lung [131]; Oropharyngeal [132]; pancreatic [133];

103

CH60

60 kDa heat shock protein, mitochondrial

Score: 762

Mass: 61016

pI: 5.70

Coverage: 39 %

Mitochondria

Up Regulated

PR

OR

PR

macromolecular assembly; stress working chaperone

Bronchus [134]; Colon [135]; leukemia [136]; prostate [137]

104

TCPQ

T‐complex protein 1 subunit theta

Score: 200

Mass: 59583

pI: 5.42

Coverage: 24 %

Cytoplasm

Down Regulated

R

OR

R

chaperone

Colon [138]; liver [139];

127

GRP75

Stress‐70 protein, mitochondrial

Score: 839

Mass: 73635

pI: 5.87

Coverage: 33 %

Mitochondria, nucleus

Down Regulated

R

PR

R

Cell proliferation; cellular aging; chaperone

Brain, Breast, colon, kidney, lung, ovarian [140]

139

GLU2B

Glucosidase 2 subunit beta

Score: 80

Mass: 59388

pI: 4.33

Coverage: 11 %

Endoplasmic reticulum

Up Regulated

OR

OR

-

Regulatory subunit of glucosidase II

Colon [138, 141]; sarcoma [142];

149

HSP74

Stress‐70 protein, mitochondrial

Score: 187

Mass: 94271

pI: 5.11

Coverage: 10 %

Cytoplasm

Down Regulated

-

OR

PR

Stress response; cell proliferation, differentiation

Breast [143]

170

HNRPC

Heterogeneous nuclear ribonucleoproteins

Score: 142

Mass: 33650

pI: 4.95

Coverage: 8 %

Nucleus

Up Regulated

-

R

OR

mRNA processing and translation

Breast [144]; colon [145]; lung [146]; pancreatic [147];

215

CALR

Calreticulin

Score: 246

Mass: 48112

pI: 4.29

Coverage: 17 %

Endoplasmic, sarcoplasmic reticulum

Up Regulated

-

R

OR

Calcium-binding chaperone

Bladder [69]; breast [148]; colon [149]; gastric [150]; glioblastoma [69]; liver [151]; ovarian [69]; pancreas [152]; prostate [153]

246

VIME

Vimentin

Score: 539

Mass: 53619

pI: 5.06

Coverage: 81 %

Cytoplasm

Up Regulated

P

OR

-

Filaments attached to nucleus and endoplasmic reticulum

Breast [154]; gastric [155]; lung [156]; pancreas [157]