High sensitivity isoelectric focusing to establish a signaling biomarker for the diagnosis of human colorectal cancer

Table 1 Summary of changes in signaling components between normal and CRC tissues

Signaling component	Normal	CRC II	CRC IV	Comment
EGFR	+	+	+	Similar levels in benign, CRC II and IV.
pAKT/AKT	+	+	+	Total levels of pAKT and AKT upregulated in CRC but pAKT/AKT ratios were similar in the different samples.
p70S6K	+	+	+	Similar levels in benign, CRC II and IV.
PLCγ1	-	+++	+ +	Low or no PLCγ1 expression in benign samples and higher levels in CRC. pPLCγ1 was not detected in any samples.
SRC pY527/SRC	+++	+	+	Low or no SRC pY418 in all samples. Lower SRC pY527/SRC ratios in CRC II and IV compared to benign.
pERK1/ERK1	+++	+	+	Reduced pERK1/ERK1 ratio in CRC II and IV compared to benign.
pERK2/ERK2	++	++	++	Similar ratios benign, CRC II and IV.
MEK1/2	+	+	+	Similar levels in benign, CRC II and IV.

+, ++ and +++ indicate different relative levels or ratios for a particular signaling component when comparing normal, CRCII and CRCIV samples, and should not be applied to compare levels/ratios between the different signaling components

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