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Fig. 5 | BMC Cancer

Fig. 5

From: High sensitivity isoelectric focusing to establish a signaling biomarker for the diagnosis of human colorectal cancer

Fig. 5

Detection of ERK1/2 total protein and phosphorylated forms by isoelectric focusing. Plots (c–e) show values after normalization to HSP70 levels. Symbols in plots: Red; KRAS mutated, green; BRAF mutated, blue; wild type (WT) with regard to KRAS and BRAF, black; unclear for KRAS and BRAF. a. Immunoblotting of selected tissue samples with antibodies against pERK1/2 and total ERK1/2 protein. Loading control β2M was same as shown in Fig. 2a. b. Representative electropherogram showing ERK1/2 total protein peaks. c. Plot of individual peak areas from ERK1 (ppERK1+ pERK1 + ERK1) analyses of normal mucosa and CRC stage II and IV (top) and of pERK1 (ppERK1 + pERK1)/ERK1 peak areas (bottom) after normalization for HSP70 run in parallel. d. Plot of normalized ERK2 (ppERK2 + ERK2) protein peaks (top) and pERK2 (ppERK2)/ERK2 (bottom). e. Plot of normalized, combined ERK1/2 total protein peaks (top) and combined pERK1/2 over total ERK1/2 peaks (bottom)

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