Fig. 6

Comparison of coverage distribution across 90 PCR-capture amplicons of both WES systems. The comparison shows a lower uniformity across the amplicons in Agilent libraries, with a higher number of low read depth regions (20 amplicons with coverage <20× vs 14 of Roche) or very high coverage (10 amplicons with coverage >80× vs 2 of Roche). Both whole exome capture systems showed a poor coverage in TP53 with 5/8 unsuccessfully covered amplicons (<20×) in each WES system. Coverage values were transformed in logarithmic scale