Fig. 2

MiR-21-mediated down-regulation of HPGD and up-regulation of proliferation. a miR-21 mimic and negative control mimic were introduced into the UM1, UM2, SCC9 Tca8113 and HeLa cells. qRT-PCR was performed to assess the expression of HPGD. b Western blot was performed to assess the expression of HPGD at protein level in UM1 cells treated with either miR-21 mimic or negative control mimic. c The expression HPGD was measured by fluorescent immunocytochemical analysis in UM1 cells treated with either miR-21 mimic or negative control mimic (Green: HPGD; Blue: DAPI nuclear staining). d The UM1 and Tca8113 cells were treated with miR-21 mimic or negative control mimic, and the cell proliferation was assessed by MTT assay. Data represents at least 3 independent triplicate experiments with similar results. * indicates p < 0.05