Fig. 2

EIF5A2 increased cells’ motility. a EIF5A2 protein level was determined in the CNE2 and HONE1 cells transduced with lenti-eIF5A2 and vector control. Tubulin or actin was used as loading control. b Representative pictures and summary of cell migration assay demonstrated that cell motility was increased in EIF5A2 overexpressed cells compared with vector control cells. c EIF5A2 was knocked down by siRNA in CNE2 and HONE1 cells. The protein level was determined by WB. Actin was used as loading control. d Representative pictures and summary of cell migration assay demonstrated that knock-down of EIF5A2 decreased cells’ motility. Original magnification: 10× objective