Fig. 2

a Immunocytochemistry showing Akt phosphorylation in U87 cells after exposure to dactolisib at doses indicated for 72 h. Upper panel: Akt phosphorylated at site T308 (FITC, green). Middle panel: Akt phosphorylated at site S473 (FITC, green). Lower panel: Total Akt-levels (FITC, green). Nuclear counterstaining: DAPI (blue). b Left: Western blots showing levels of pAkt (T308), pAkt (S473) andtotal Akt in U87 cells exposed to dactolisib at doses indicated for 72 h. Right: Densitometric assessment of western blot, showing relative change in phosphorylation. c Left: Western blot showing levels of pAkt (T308), pAkt (S473) and total Akt in P3 cells exposed to dactolisib at doses indicated for 72 h. Right: Densitometric assessment of western blot, showing relative change in phosphorylation. Error bars represent s.d. of Error bars represent s.d. of three (a and b) and two (c) independent experiments. *P <0.05, **P < 0.01, ***P < 0,001