Fig. 4
From: The transcriptional regulator TBX3 promotes progression from non-invasive to invasive breast cancer
TBX3 overexpression increases migration and invasion of 21NT cells. a, b TBX3 overexpression increases migration using a transwell migration assay. 21NT cell transfectants were placed in the upper chamber of a transwell migration system with media containing 1 % BSA and allowed to migrate towards αHE10F chemoattractant in the bottom chamber for 22 h at 37 °C. Stable transfectants of both TBX3 isoforms showed an increase in migration using the transwell system. Scale bars represent 100 μm. Results are quantified in panel (b). c, d TBX3 overexpression increases invasion using a transwell invasion system. 21NT cell transfectants were placed in the upper chamber of a transwell invasion system with media containing 1 % BSA and allowed to invade towards αHE10F chemoattractant in the bottom chamber for 22 h at 37 °C. Stable transfectants of both TBX3 isoforms showed an increase in invasion using the transwell system. Scale bars represent 100 μm. Results are quantified in panel (d)