Fig. 1

TBX3 isoform 1 and TBX3 isoform 2 are differentially expressed in the 21T cell lines. a Total TBX3 is differentially expressed in the 21T cell lines. Primers for total TBX3 mRNA were used to detect total TBX3 levels in the 21T cell lines. TBX3 is significantly higher in the 21MT-1 cells compared to the 21PT and 21NT cell lines and there is no significant difference in TBX3 expression between the 21PT and 21NT cells. b TBX3 isoform 1 is differentially expressed in the 21T cell lines. Primers specific for TBX3iso1 were used to detect TBX3iso1 levels in the 21T cell lines. TBX3iso1 is significantly higher in the 21MT-1 cells compared to the 21PT and 21NT cell lines and there is no significant difference in TBX3iso1 expression between the 21PT and 21NT cells. c TBX3 isoform 2 is differentially expressed in the 21T cell lines. Primers specific for TBX3iso2 were used to detect TBX3iso2 levels in the 21T cell lines. TBX3iso2 is significantly higher in the 21MT-1 cells compared to the 21PT and 21NT cell lines and there is no significant difference in TBX3iso2 expression between the 21PT and 21NT cells. d, e Total TBX3 protein is differentially expressed in the parental 21T cell lines. An antibody recognizing both isoforms of TBX3 was used to assess the relative abundance of TBX3 within the 21T cell lines. Total TBX3 protein expression is significantly higher in the 21MT-1 cells compared to the 21PT and 21NT cell lines and there is no significant difference in total TBX3 protein expression between the 21PT and 21NT cells. Means were analyzed using one-way ANOVA followed by Tukey’s post hoc test and p < 0.05 was considered statistically significant; ** p < 0.01. Error bars indicate Standard Error. Results are representative of at least three independent experiments