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Fig. 7 | BMC Cancer

Fig. 7

From: Histone deacetylase inhibitors induce invasion of human melanoma cells in vitro via differential regulation of N-cadherin expression and RhoA activity

Fig. 7

Rho implication in HDACi induced invasion. a Active Rho pull down assay in A375 melanoma cells treated for 48 h with HDACi. Butyrate, TSA, Valproic acid and Vorinostat promoted active Rho downregulation. b Collagen invasion assay using Rho inhibitor C3T and ROCK inhibitor Y27. c HT −144 cells transfected with dominant negative for RhoA-T19N plasmid. Both inhibitors emulated the effect of HDACi on HT-144 invasion, as well as Rho downregulation by transfection. d Transfection efficiency measured as GFP transfected cells in cytometry. e GFP transfected cells as seen with fluorescence microscopy. *** Statistically significant difference from control, p < 0.001

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