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Fig. 2 | BMC Cancer

Fig. 2

From: Histone deacetylase inhibitors induce invasion of human melanoma cells in vitro via differential regulation of N-cadherin expression and RhoA activity

Fig. 2

Invasion assays. a Matrigel invasion assay with the A375 cell line. Cells were treated with butyrate, TSA, Valproic acid and Vorinostat during 24 h. All HDACi significantly increased melanoma cell invasion. b Confocal microscopy of the transwell membrane with A375 cells stained with Hoechst, corresponding to control (left) and butyrate treatment (right) from Matrigel invasion assay. Scale bar, 500 μm. c Type I collagen invasion assay with HT-144 cell line. Cells were treated with the same agents as in (a) for 24 h. Butyrate, TSA and Vorinostat induced a significant increase on invasion, whereas Valproic acid had no effect. d Contrast phase microscopy of HT-144 cells on collagen gel, corresponding to control (left) and vorinostat (right) after 24 h treatment. Scale bar, 50 μm. Statistically significant differences from control; ***p < 0.001, ** p < 0.01 and * p < 0.05. Abbreviations: But, butyrate; TSA, Trichostatin A; VPA, Valproic acid; Vor, Vorinostat

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