Fig. 4
From: Polo-like Kinase 1 as a potential therapeutic target in Diffuse Intrinsic Pontine Glioma
Small molecule inhibition of PLK1 induces DNA damage in DIPG cells. a DIPGIV cells were treated with IC50 dose of BI 6727 for 6 or 24 h and analyzed for phospho-histone H2AX (γH2AX) by immunofluorescent imaging. Representative images are shown; blue-DAPI nuclei stain and green- γH2AX. b Quantification of the percent of γH2AX positive cells identified in (A) normalized to DAPI. Percent ± SEM is shown. c DIPG4 cells were treated with IC25 and IC75 doses of BI 6727 for 24 h and expression of the DNA damage response proteins p53, p21, RAD51, and phospho-γH2AX were evaluated by western blot analysis